domingo, 14 de abril de 2013

Bioengineered kidney makes urine


Scientists at the Massachusetts General Hospital in Boston have created a bioengineered kidney that can be transplanted back into a rat, where it begins making urine. Nature Medicine takes a look at how growing organs in the laboratory might someday be the solution to help people with end-organ failure.

Regeneration and experimental orthotopic transplantation of a bioengineered kidney

Nature Medicine
Published online

Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit 
  • perfusion, 
  • filtration, 
  • secretion, 
  • absorption and 
  • drainage 
of urine. 

We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.

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