Mostrando entradas con la etiqueta Epigenética. Mostrar todas las entradas
Mostrando entradas con la etiqueta Epigenética. Mostrar todas las entradas

martes, 25 de febrero de 2014

Fellowships in Biochemistry, Cell Biology, Developmental Biology, Epigenetics, Immunobiology and Molecular Biology. Max Planck Institute





PhD overview

Our programme provides outstanding students with excellent research-oriented interdisciplinary training in Biochemistry, Cell Biology, Developmental Biology, Epigenetics, Immunobiology and Molecular Biology. We train students to become self-reliant and to acquire and utilize the knowledge necessary for their research project. Students have the chance to interact with experts in various fields both to extend their scientific knowledge and to learn critical thinking as well as acquire problem-solving skills.

The IMPRS-MCB fellows have 3 to 4 years to obtain their degree. Most of this time they work on their individually supervised research project. In addition to the experimental part all fellows have a possibility and are obliged to participate in curricular activities of the programme. These are:
  • scientific and soft skills courses offered by the programme (see Curriculum)
  • institute seminars
  • PhD retreat
  • supervision of junior students (master students or summer trainees)
  • conference attendance
  • career evenings

Our students are funded during the whole time of their PhD (3-4 years). The payment is sufficient for monthly living costs in Freiburg to be covered. There are no tuition fees to be paid.

Graphical overview of the curricular activities during your PhD
Figure Legend: Orange - activities related to research and the PhD project; Blue - courses; Green - scientific conference/retreats

ORIGINAL: Max Planck Institute

jueves, 23 de enero de 2014

Epigenetics: A Timeline

Researchers are clarifying epigenetic intricacies such as 
  • missing heritability, 
  • disease markers, 
  • methylated proteins, and 
  • imprinted genes. 
But how did we get here? Learn about the history of epigenetics in this timeline spanning 130 years



ORIGINAL: GEN News
Jan 14, 2014

jueves, 1 de agosto de 2013

Portable Brain-Scan Headsets: 4 Incredible Applications

ORIGINAL: NatGeo
Brian Handwerk. for National Geographic
August 1, 2013




New technology is moving brain research outside the lab and into the real world.



The Emotiv Insight brain-scanning headset may help scientists understand the mind on the go. Image courtesy Emotiv Lifesciences


Our brain controls what we think, feel, and do, but scientists have a limited capability to watch it at work outside the lab. National Geographic Emerging Explorer Tan Le hopes to change that while, in the process, fighting neurological disorders, enhancing learning, and even helping the disabled move things in the physical world with the power of the mind.

Emotiv Lifesciences, the company Le co-founded, produces portable, high-resolution EEG (electroencephalogram) brain-scanning headsets that Le hopes will open new windows on the complex functioning of our brain. On August 1, Emotiv unveiled Emotiv Insight, a faster, next-generation wireless brain scanner that collects real-time data on the wearer's thoughts and feelings and delivers it directly to a computer, phone, or other device through Android, iOS, OSX, Linux, and Windows platforms.



Tan Le, Photograph from Australia Unlimited/National Geographic
Le hopes the product, which costs $199, can further democratize brain research and help scientists gather more data. Using the EEG headsets, she says, people around the world can study brains under conditions and stimuli as varied as those we encounter in everyday life—because subjects can wear the headset while doing everyday tasks.

"The idea is to empower us all to understand more about ourselves," Le said. "That's really the mystery of the mind."

She added, "It's all very, very personal. Sure, there are some commonalities in the way the brain functions. But what we know now with epigenetics [the study of how the expression of heritable traits is modified by environmental influences] is that every learning experience, every activity we undertake, actually affects our [neural] networks. Your brain yesterday is different than your brain today."

An EEG records the electrical fluctuations in the brain and tracks changes in activity as neurons fire when you are engaged in a cognitive task. It's a time-tested process, but traditionally its use has been limited to the lab and a subset of people because it's relatively costly and time-consuming.

What's needed instead, Le stressed, is as much data as possible on as many brains as possible.

"Until recently there's not been any sort of concentrated effort to collect EEG recordings on well individuals, and that really is an essential part of any sort of background study into abnormalities," Le said. "It's a classic case of science being very skewed toward studying the problem set."

So if we're interested in epilepsy we study patients with epilepsy. If we're interested in Alzheimer's we study patients with Alzheimer's, Le noted.

But with no idea what the norm is, she added, correlations or features that appear in these brains may be present in non-afflicted people as well.

Emotiv hopes to help create a massive digital repository of brain-scan information, as well as a platform for sharing brain data with interested parties around the world. With that information, researchers could send out experiments online and collect data from a wide range of subjects who wear the headsets while performing all types of directed tasks.


"It used to be that you sat in your lab and worked kind of independently of other people," said Kevin Whittingstall, the Canada Research Chair in Neurovascular Coupling and a professor at the University of Sherbrooke. "What we've realized is that the brain is so complex we need to start grouping together data sets to paint a better picture."

"What I think is very promising with the Emotiv system is that the hardware remains constant," added Whittingstall, who has no connection to the project. "With one hospital using one EEG system and another using another system, the electrode positions might be different, for example, and it's hard to integrate the data." (Read "Beyond the Brain" from National Geographic magazine.)

Here are four applications of the portable brain scanners that Le says are already beginning to take shape:

1. Moving Things With the Mind
The EEG headset has helped paralyzed patients control an electric wheelchair and make music via computer using only the power of their minds, said Le.

"The hope is that it's going to be more of a democratizing force, so that whether you are able-bodied or not you're still going to be able to communicate and interact with your world in a meaningful way," Le said during a recent trip to Washington, D.C., for the National Geographic Explorers Symposium. "And we're starting to see the first signs of that."

When patients think of an action—verbalizing a word, for example—the headset can record the brain patterns for that action into a computer via a wireless connection. Then, when the wearer repeats the action, the computer can perform it—allowing or facilitating communication, for example, among those who had lost some or all of that ability.

"I think it speaks to the power of software and algorithms to decipher and interpret electrical signals from the brain," Le said. "When you can start to interpret what's going on in the brain, you can extract unique features that then can help you use them as a command to trigger different events in a device or an application on some sort of computing platform."

The human-machine interface can be used for things as trivial as playing a video game, or as life changing as operating a prosthetic limb—and today's achievements represent only the tip of the iceberg, said Le.

2. Diagnosing Disease
Le stresses that because we lack a large, easily accessible database of "normal" brain data, we're likely missing opportunities to identify and track the causes of brain ailments from their earliest stages—when intervention might be less dramatic and more successful.

"A lot of these conditions are developmental in nature, meaning that you don't get Alzheimer's [or autism] overnight," she said.

Historically, Whittingstall added, most of our information regarding brain function was obtained by studying how damage to one particular area was linked to a cognitive deficit, such as language or memory impairment.

"With EEG, we can now start to non-invasively map out brain function so it enables you to record data from many subjects and improves the statistical power to detect the tiniest differences between experimental conditions," he added.

Le hopes the headset will not only help identify neurological conditions and study their progressions in the ever-changing brain, but also enable intervention.

"The brain is plastic; it's very capable of change, so if it's going down a route that we don't want it to go down, then let's do something about it and fine-tune how we intervene based on feedback from the brain itself," she said. (Video: Visit the Brain Bank at Harvard.)

3. Making Learning Easier
With EEG technology becoming more affordable, scientists and citizens alike can get a more complete picture of how each individual brain operates in real-world situations, ranging from social interactions to studying to intense physical activity, said Le.

"People are more and more interested in quantifying their physical health, and I think we're going to start seeing people more interested in quantifying their cognitive, behavioral, and mental health," Le said.

This window on how the individual brain works could also inspire personally tailored applications to help it learn better. "The value to the individual is that you can start to put together some sort of productivity profile for yourself," said Le. "When am I optimized to do some sort of creative brainstorm work?" she added, as an example.

Whittingstall agreed that such learning boosts could be part of the near-term future. "If you can monitor the brain as someone learns a language, for example, you might correlate their ability to learn through changes in their brain waves," he said.

"However, being able to actually put your brain into that state that's optimized for learning might be further off," he cautioned. (Related: "How Your Brain Cleans Itself—Mystery Solved?")

4. Organizing Data by Thoughts and Emotions
Le suggests that brain-scan information could one day be used to help people organize the avalanche of data, videos, images, audio, and other media that is steadily mounting.

"The first time you view an image [of something you experienced], you're going to have a very strong visceral response because you are reliving that moment," she explained, pointing to the example of watching a video of your child taking her first steps.

Because such images can have a strong emotional cue, there may be a way to label them with some kind of personalized emotional tag based on a brain scan, Le suggests. The concept could allow each of us to build a library of cherished video or images that are organized not just by date or location, but also by the emotional descriptions of what each of them produce in our own brains.

All these applications, exciting as they seem, represent a technology that's still in its infancy—and no one knows where it may lead. "There's no way to tell what the killer app is," Le said.

"The biggest job is to try to get as many people excited about the technology as possible so they can play with it, work with it, and expand it in their own areas of interest and their own passions," she said. "Hopefully we can find some great innovations as a result."

What you can do with a little Insight…
Optimize your brain fitness and performance.
Measure you or your family's cognitive health and wellbeing
Create amazing applications with our APIs and analysis tools.

Tan Le: A headset that reads your brainwaves
http://www.ted.com Tan Le's astonishing new computer interface reads its user's brainwaves, making it possible to control virtual objects, and even physical electronics, with mere thoughts (and a little concentration). She demos the headset, and talks about its far-reaching applications.

sábado, 27 de julio de 2013

New Hope for Hormone Resistant Breast Cancer

ORIGINAL: Science Daily

July 22, 2013 — A new finding provides fresh hope for the millions of women worldwide with estrogen receptor positive breast cancer. Australian scientists have shown that a specific change, which occurs when tumours become resistant to anti-estrogen therapy, might make the cancers susceptible to treatment with chemotherapy drugs.

Seventy percent of breast cancer patients have estrogen receptor positive cancer, and most patients respond well to anti-estrogen therapies, for a few years at least. Within 15 years, however, 50% will relapse and eventually die from the disease.

Dr Andrew Stone, Professor Susan Clark and Professor Liz Musgrove, from Sydney's Garvan Institute of Medical Research, in collaboration with scientists from Cardiff University, have demonstrated that the BCL-2 gene becomes epigenetically 'silenced' in resistant tumours. This process is potentially detectable in the blood, providing a diagnostic marker. Their findings are now online in the international journal Molecular Cancer Therapeutics.

Epigenetics involves biochemical changes in our cells that directly impact our DNA, making some genes active, while silencing others. Epigenetic events include DNA methylation, when a methyl group -- one carbon atom and three hydrogen atoms -- attaches to a gene, determining the extent to which it is 'switched on' or 'switched off'.

Dr Stone and colleagues have shown in human disease, as well as in several different cell models, that BCL-2 is silenced in estrogen-resistant tumours by DNA methylation.

"The main purpose of the BCL-2 gene is to keep cells alive, so when the gene is silenced, cells become more vulnerable to chemotherapy," said Dr Stone.

"The next step will be to test our findings in clinical studies. We propose that if the BCL-2 gene is silenced, patients with estrogen receptor positive breast cancer would benefit from combination therapy. In other words, tamoxifen could be used in combination with a chemotherapy drug, to kill off vulnerable tumour cells."

"Excitingly, this is something that could be implemented into clinical practice very quickly, since the technology now exists to profile methylation of BCL-2 in all patients -- both estrogen responsive and estrogen resistant patients. In addition, the proposed chemotherapy drugs are already in use."

"If such a test were to be implemented, we believe it could help patients much earlier -- hopefully shutting down tumours at an early stage."

Estrogen is a hormone produced by all women, and drives reproduction, menstruation and menopause. Many breast cells have estrogen receptors on their surface. When one of those cells becomes malignant and starts to multiply, an estrogen receptor positive tumour develops. Typically these tumours are fed by high concentrations of estrogen in the bloodstream, triggered by circumstances such as late first pregnancy, not breastfeeding, or too much body fat.

At present, there are three different therapeutic strategies for estrogen receptor positive breast cancer: 
  1. blocking the body's production of estrogen with aromatase inhibitors; 
  2. preventing estrogen from 'binding' to the estrogen receptor by 'binding' the drug tamoxifen instead; and 
  3. destroying the estrogen receptor completely.
Tamoxifen, now the gold standard treatment, became available in the 1970s.

Endocrine therapies, including tamoxifen, are inexpensive and well-tolerated, and are usually given for 5 years. Over time, however, many tumours become resistant to therapy. When one form of treatment starts to fail, a second is put in place, and then a third. Response times shrink, and tumours start to spread to other parts of the body, usually the brain, lungs, liver and bone.

At this late stage, there is little that can be done to help someone, as the brain, lungs, liver and bone are parts of the body that cannot be biopsied or operated upon.

domingo, 20 de enero de 2013

Biology Basics: What’s the deal with Epigenetics?

ORIGINAL: OBR Review
Written by Brian Abraham
January 17, 2013

Photo credit: © WGBH Educational Foundation
Why do we care?
With few exceptions, namely functionally variable regions in the immune system and in disease, all of a person’s cells have the same DNA sequences and genes. Yet the variety of cells in terms of shape and function is vast. How is it that a muscle cell and a blood cell have the same genes yet do such strikingly different things?

In short, not every gene is being used or transcribed by all cell types: There are certain genes that are essential for blood cells that muscle cells don’t express (transcribe) or use. For example, genes making proteins that recognize foreign bodies would be useful for an immune blood cell but would be a waste of a muscle cell’s energy. The burgeoning fields of epigenetics and epigenomics investigate these patterns of on/off genes and what regulates their expression. Epigenomics refers to the genome-wide application of epigenetics, which generally refers more to single-gene analyses.

Where does it apply?
“Epigenetic” as an adjective refers to heritable traits that are not changes to the DNA sequence itself. Heritability can be across both cell divisions as well as trans-generational. When a fat cell replicates and both daughter cells retain fat cell traits, inheritance is referred to as divisional/replication inheritance. When offspring retain traits of their parents, the inheritance is termed trans-generational inheritance.

A famous example of such trans-generational epigenetic inheritance is the Dutch Hongerwinter. In 1944, the Dutch in areas of the Nazi-occupied Netherlands were starving as the result of a blockade. Fetuses that developed in the wombs of mothers surviving the famine showed a number of characteristic traits of nutrient deprivation themselves, including glucose sensitivity. Later biochemical analyses demonstrated epigenetic marks on genes regulating insulin-like growth factors– characteristics of nutrient deprivation– despite no change in the DNA of these genes. Further tests of second-generation offspring may show that these marks and traits acquired in response to starvation are inherited across multiple generations.

Before Darwin’s famous description of evolution by natural selection, Jean-Baptiste Lamarck proposed the idea that traits acquired by the parent can be passed down to offspring. For instance, a giraffe’s neck is long because its parents had to stretch to reach higher trees. When On the Origin of Species elegantly explained population shifts by survival, the idea of heritability of acquired traits lost popularity. The theory was further buried when Mendelian genetics demonstrated the mechanism of genetic inheritance. It has lain dormant until a recent resurgence in interest implicating epigenetics as a plausible explanation for trans-generational inheritance of acquired traits.

How does it work?
Roughly 3 meters of DNA sequence is stored inside every nucleated human cell; the condensation and packaging of the DNA sequence is essential to regulation of gene expression. In general, uncondensed genes are expressed more readily than condensed genes. Following is a discussion of the various methods by and degrees to which genes are condensed into “chromatin”– the name given to the DNA/condensing protein complex.